WAKO過氧化氫特異性熒光探針

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WAKO過氧化氫特異性熒光探針

品牌： Wako(和光純藥)
貨號： 028-17811
CAS號： 728912-55-8
供應(yīng)商： 和光純藥株式會社
規(guī)格： 1mg
過氧化氫特異性熒光探針BES-H2O2-Ac / BES-H 2O2
? 比二氯熒光黃 (DCFH) 有更高的特異性
? 可動態(tài)監(jiān)測活細胞內(nèi)的過氧化氫
? BES-H2O2-Ac 可穿透細胞膜，無需打孔
? 極易溶于水，可制成水溶液使用
? 可用于熒光顯微鏡、流式細胞儀

BES-So-AM：使用舉例

圖1）是Jurkat T細胞在含33μM BES-So-AM的培養(yǎng)基中37°C培養(yǎng)1小時，使探針進入細胞，然后添加4mM丁酸（O2?-誘導劑），37°C培養(yǎng)1小時；圖2）是Jurkat T細胞在含33μM BES-So-AM的培養(yǎng)基中37°C培養(yǎng)1小時，使探針進入細胞，然后不添加丁酸，37°C再培養(yǎng)1小時；圖3）是Jurkat T細胞在含33μM BES-So-AM的培養(yǎng)基中37°C培養(yǎng)1小時，然后添加5mM的丁酸，繼續(xù)培養(yǎng)一個小時。參考文獻
1) Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 239 (2004).
2) Maeda, H. et al.: Chem. Pharm. Bull., 49, 294 (2001).1. Features

?Much higher selectivity compared to conventionally-used DCFH probes?Responds specifically to hydrogen peroxide, enabling detection of intracellular hydrogen peroxide activity?BES-H2O2-Ac is cell membrane permeable?Superior water solubility allows use with aqueous solutions?Can be used in flow cytometry

2. Application

H2O2-produced stimulation No H2O2-produced stimulation
Fluorescent images
Phase-contrast images
Fluorescent images of Jurkat T cells with BES- H2O2 -Ac and the same-fi eld phase-contrast images
Jurkat T cells were cultured in a medium with 50μM BES-H2O2-Ac for 1 hour. Then, one group of them was cultured in a medium with 5 mM butyric acid (H2O2-produced stimulation), whereas the other in a medium without butyric acid (No H2O2– produced stimulation), each for 1 hour. (Courtesy: Professor Hatsuo Maeda, PhD., School of Pharmacy, Hyogo Univ. of Health Sciences)

 

3. References

  1. “Fluorescent probe for hydrogen peroxide based on a non-oxidative mechanism”, Maeda, H. et al.: Angew. Chem. Int. Ed., 43, 2389-91 (2004).
  2. “Hydrogen peroxide-induced deacetylation of acetyl resorufin as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase”, Maeda, H. et al.: Chem. Pharm. Bull., 49, 294-8 (2001).

4. Products List

< All products are for Cellbiology; ? Keep at room temperature >

Cell Permeability Product Name Package Size Wako Catalog No.
Thiol/Selenol
selective probe
x
 BES-Thio
1 mg
025-15481
H2O2 Probes
o
<Detection of
cell-derived H2O2
 BES-H2O2-Ac
1 mg
028-17811
x
 BES-H2O2 ? (Cell-impermeant)
1 mg
021-16201
Superoxide
selective probe
o
<Detection of
cell-derived superoxide>
 BES-So-AM
1 mg
021-17801
x
 BES-So ? (Cell-impermeant)
1 mg
028-16211

說明書下載：

http://www.wako-chem.co.jp/english/labchem/product/life/BES-H2O2/pdf/WPUb1_p15-7.pdf