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BCECF
BCECF
- 細(xì)胞內(nèi)蛍光プローブ
- 細(xì)胞內(nèi)蛍光プローブ
細(xì)胞內(nèi)pH測定試薬
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製品コードB031 BCECF
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CAS番號85138-49-4
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化學(xué)名2′,7′-Bis(carboxyethyl)-4 or 5-carboxyfluorescein
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分子式?分子量C27H20O11=520.44
| 容 量 | メーカー希望 小売価格 |
富士フイルム 和光純薬 |
|---|---|---|
| 5 mg | ￥14,600 | 345-05184 |
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マニュアル
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プロトコル
細(xì)胞內(nèi) pH を測定したい 
技術(shù)情報
注意事項
?本製品を粉末の狀態(tài)で取り出し使用する場合、性狀の性質(zhì)上、靜電気等の要因で容器內(nèi)に付著し、取り出しにくい場合があります。
?容器內(nèi)に付著し、取り出せなかった粉末に関しては、使用する溶媒を容器に入れ、溶かし出して使用してください。
參考文獻(xiàn)
1) R. A. Steinhardt and D. Mazia, "Development of K+-conductance and Membrane Potentials in Unfertilized Sea Urchin Eggs After Exposure to NH4OH", Nature, 1973, 241, 400.
2) T. J. Rink, R. Y. Tsien and T. Pozzan, "Cytoplasmic pH and Free Mg2+ in Lymphocytes", J. Cell Biol., 1982, 95, 189.
3) A. M. Paradiso, R. Y. Tsien and T. E. Machen, "Na+ -H+ Exchange in Gastric Glands as Measured with a Cytoplasmic-trapped, Fluorescent pH Indicator", Proc. Natl. Acad. Sci. USA, 1984, 81, 7436.
4) S. Grinstein, B. Elder and W. Furuya, "Phorbol Ester-induces Changes of Cytoplasmic pH in Neutrophils: Role of Exocytosis in Na+ – H+ Exchange", Am. J. Physiol., 1985, 248, C379.
5) G. B. Zavoico, E. J. Cragoe and M. B. Feinstein, "Regulation of Intracellular pH in Human Platelets", J. Biol. Chem., 1986, 261(28), 13160.
6) G. R. Bright, W. Fisher, J. Rogowska and L. Taylor, "Fluorescence Ratio Imaging Microscopy: Temporal and Spatial Measurements of Cytoplasmic pH", J. Cell Biol., 1987, 104, 1019.
7) C. Aalkjaer and E. J. Gragoe Jr, "Intracellular pH Regulation in Resting and Contracting Segments of Rat Mesenteric Resistance Vessels", J. Physiol., 1988, 402, 391.
8) K. Tsujimoto, M. Semadeni, M. Huflejt and L. Packer, "Intracellular pH of Halobacteria Can Be Determined by the Fluorescent Dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein", Biochem. Biophys. Res. Commun., 1988, 155, 123.
9) M. A. Kolber, R. R. Quinones, R. E. Gress and P. A. Henkart, "Measurament of Cytotoxicity by Target Cell Release and Retention of the Fluorescent Dye Bis-carboxyethyl-carboxyfluorescein(BCECF)", J. Immunol. Methods, 1988, 108, 255.
10) H. Harada, Y. Kanai, M. Anzai and Y. Suketa, "cAMP Activates Cl–/HCO3– Exchange for Regulation of Intracellular pH in Renal Epithelial Cells", Biochim. Biophys. Acta, 1991, 1092, 404.
11) C. C. Freudenrich, E. Murphy, L. A. Levy, R. E. London and M. Lieberman, "Intracellular pH Modulates Cytosolic Free Magnesium in Cultured Chicken Heart Cells", Am. J. Physiol., 1992, 262(4), C1024.
12) K. Khodakhah and D. Ogden, "Functional Heterogeneity of Calcium Release by Inositol Triphosphate in Single Purkinje Neurones, Cultured Cerebellar Astorocytes, and Peripheral Tissues", Proc. Natl. Acad. Sci. USA, 1993, 90, 4976.
13) G. Boyarsky, C. Hanssen and L. A. Clyne, "Superiority of in vitro Over in vivo Calibrations of BCECF in Vascular Smooth Muscle Cells", FASEB J., 1996, 10, 1205.
14) S. A. Weston and C. R. Parish, "New Fluorescent Dyes for Lymphocyte Migration Studies Analysis by Flow Cytometry and Fluorescent Microscopy", J. Immunol. Methods, 1990, 133, 87.
15) L. S. D. Clerck, C. H. Bridts, A. M. Mertens, M. M. Moens and W. J. Stevens, "Use of Fluorescent Dyes in the Determination of Adherence of Human Leucocytes to Endothelial Cells and the Effects of Fluorochromes on Cellular Function", J. Immunol. Methods, 1994, 172, 115.
取扱條件
| 性狀： | 本品は、橙色～赤茶褐色粉末でメチルアルコールに溶ける。 |
|---|---|
| 純度(HPLC)： | 85.0％ 以上 |
| メチルアルコール溶狀： | 試験適合 |
| 蛍光スペクトル： | 試験適合 |
| IRスペクトル： | 試験適合 |
| NMRスペクトル： | 試験適合 |
| 1.保存方法：遮光 |
