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DiBAC4(3)
DiBAC4(3)
- 細(xì)胞內(nèi)蛍光プローブ
膜電位測定試薬
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製品コードD545 DiBAC4(3)
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CAS番號70363-83-6(free acid)
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化學(xué)名Bis(1,3-dibutylbarbituric acid)trimethine oxonol, sodium salt
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分子式?分子量C27H39N4NaO6=538.61
| 容 量 | メーカー希望 小売価格 |
富士フイルム 和光純薬 |
|---|---|---|
| 25 mg | ￥35,000 | 349-90231 |
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マニュアル
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プロトコル
膜電位を測定したい 
參考文獻(xiàn)
1) D. E. Epps, M. L. Wolfe and V. Groppi, "Characterization of the Steady-state and Dynamic Fluorescence Properties of the Potential-sensitive Dye Bis-(1,3-dibutylbarbituric acid)trimethine oxonol(Dibac4(3)) in Model Systems and Cells", Chem. Phys. Lipids, 1994, 69, 137.
2) T. Braner, D. F. Hulser and R. J. Strasser, "Comparative Measurements of Membrane Potentials with Microelectrodes and Voltage-sensitibe Dyes", Biochim. Biophys. Acta, 1984, 771, 208.
3) T. T. Rohn, A. Sauvadet, C. Pavoine and F. Pecker, "Xanthine Affects [Ca2+]i and Contractile Responses of Ventricular Cardiocytes to Electrical Stimulation", Am. J. Physiol., 1997, 273, C909.
4) D. J. Mason, R. Allman, J. M. Stark and D. Lloyd, "Rapid Estimation of Bacterial Antibiotic Susceptibility with Flow Cytometry", J. Microsc., 1994, 176, 8.
5) U. Langheinrich and J. Daut, "Hyperpolarization of Isolated Capillaries from Guinea-pig Heart Induced by K+ Channel Openers and Glucose Deprivation", J. Physiol., 1997, 502, 397.
6) V. Dall'Asta, R. Getti, G. Orlandini, P. A. Rossi, B. M. Rotoli, R. Sala, O. Bussolati and G. C. Gazzola, "Membrane Potential Changes Visualized in Complete Growth Media through Confocal Laser Scanning Microscopy of bis-Oxonol-loaded Cells", Exp. Cell Res., 1997, 231, 260.
7) K. S. Schroeder and B. D. Neagle, "FLIPR: A New Instrument for Accurate, High Throughput Optical Screening", J. Biomol. Screening, 1996, 1, 75.
取扱條件
| 性狀： | 本品は、赤橙色～赤色粉末である。 |
|---|---|
| 純度(HPLC)： | 98.0％ 以上 |
| ジメチルスルホキシド溶狀： | 試験適合 |
| モル吸光係數(shù)： | 140,000 以上(493 nm付近) |
| IRスペクトル： | 試験適合 |
| 1.保存方法：冷蔵 |
