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Glucose Dehydrogenase (FADGDH-AD) 葡萄糖脫氫酶
- 臨床檢測用酶
The enzyme is useful for the determination of blood glucose and for continuous glucose monitoring (CGM) sensor.
| 由來 | recombinant Aspergillus sojae |
|---|---|
| 系統(tǒng)名稱 |
D-Glucose : acceptor 1-oxidoreductase |
| EC編號 | 1.1.5.9 |
| 反應(yīng)式 |
D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
規(guī)格
| Appearance | yellow to brown lyophilizate |
|---|---|
| Activity | ≧700 U/mg |
| Contaminants | NAD glucose dehydrogenase <0.01 U/U% |
| Hexokinase <0.01 U/U% | |
| α-glucosidase <0.01 U/U% | |
| β-glucosidase <0.01 U/U% | |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 90 kDa (SDS-PAGE) |
|---|---|
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 6.4×10-2M (D-glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 2.5–9.5 |
| Optimum temperature | 45℃ |
| Thermal stability (liquid form) | below 60℃ |
| Thermal stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Mn2+, Ag+ |
| Specificity | D-glucose (100%), maltose (0.2%), D-xylose (0.9%), D-galactose (0.8%) sucrose (<0.1%), D-mannose (0.4%) 2-deoxy-D-glucose (23.5%) |
適用類型
FADGDH-AD is useful for the determination of D-glucose in clinical analysis and continuous glucose monitoring (CGM) meter for diabetes patients. The FADGDH-AD was developed as an oxygen-insensitive alternative to Glucose oxidase (GOD) with its high stability for the applications such as bioelectrodes in glucose sensing device and glucose enzymatic fuel cells.
Line-up
- Glucose Dehydrogenase (FADGDH-AB)
參考文獻
Satake R, Ichiyanagi A, Ichikawa K, Hirokawa K, Araki Y, Yoshimura T, Gomi K (2015)
Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae
Biosci Bioeng., 120, 498-503
Masakari Y, Hara C, Araki Y, Gomi K, Ito K (2020)
Improvement in the thermal stability of Mucor prainii-derived FAD-dependent glucose dehydrogenase via protein chimerization
Enzyme Microb Technol., 132, 109387, doi: 10.1016/j.enzmictec.2019.109387.
https://doi.org/10.1016/j.enzmictec.2019.109387, (cited 2020-07-02)
- CONTACT / SAMPLE
- 產(chǎn)品單頁
- SDS