上海金畔生物科技有限公司代理龜甲萬酶制劑全線產(chǎn)品，歡迎訪問官網(wǎng)了解更多信息。
The enzyme is useful for the determination of uric acid in clinical analysis.
| 由來 |
recombinant E. coli |
| 系統(tǒng)名稱 |
Urate : oxygen oxidoreductase
|
| EC編號 |
1.7.3.3 |
| 反應(yīng)式 |
Urate + H2O + O2 →→→ Unidentified products + H2O2
↓
CO2 + Allantoin (racemic mixture)
|
規(guī)格
| Appearance |
light brownish lyophilizate |
|
| Activity |
≧4.0 U/mg |
|
| Contaminants |
Catalase ≦1.0 U/U% |
|
| Stabilizer |
sucrose, citrate, ethylenediaminetetraacetic acid (EDTA) |
|
| Storage condition |
below -20℃ |
|
特性
| Molecular weight |
ca. 90 kDa (gel filtration) |
| Structure |
2 subunits of 35 kDa (SDS-PAGE) |
| Michaelis constant |
1.1×10-5M (uric acid) |
| pH Optimum |
8.5 |
| pH Stability |
7.0–11.0 |
| Optimum temperature |
45℃ |
| Thermal stability |
below 55℃ |
| Stability (liquid form) |
stable at 37℃ for at least ten days |
| Stability (powder form) |
stable at 30℃ at least three weeks |
| Inhibitors |
Hg2+，Ag+ |
適用類型
The enzyme is useful for the determination of uric acid in clinical analysis.
參考文獻(xiàn)
Koyama, Y. et al., J. Biochem., 120, 969–973 (1996).
