| 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 產(chǎn)品規(guī)格 | 產(chǎn)品等級(jí) | 產(chǎn)品價(jià)格 |
| BIC-BCR10-00001 | CellEase??Tissue?II? ?DNA提取試劑CellEase???組織II |
見本頁 | – | – |
| BIC-BCR10-00002 | CellEase?Bacteria?II? ?DNA提取試劑CellEase???細(xì)菌II |
見本頁 | – | – |
| BIC-BCR10-00003 | CellEase??Meat?II? ?DNA提取試劑CellEase???肉II |
見本頁 | – | – |
| BIC-BCR11-00001 | CellEase??Blood? ?DNA提取試劑CellEase?血液 |
見本頁 | – | – |
| BIC-BCR11-00002 | CellEase??Plant? ?DNA提取試劑CellEase???植物 |
見本頁 | – | – |
CellEase? II DNA提取試劑
DNA extraction reagent : CellEase? II series
◆原理
此款試劑盒用于細(xì)胞內(nèi) DNA 提取，操作極為簡(jiǎn)便，提取的 DNA 可直接用于下游PCR 操作，無需純化步驟，僅需 9 分鐘。CellEase? II 跟其他產(chǎn)品相比無需洗脫，更可提高回收效率。方法簡(jiǎn)單，只需將樣品與 CellEase A和B混合，并進(jìn)行2步溫控孵育即可。
該試劑盒可從動(dòng)物組織、微生物、植物細(xì)胞等樣品中制備 DNA。該試劑盒不僅用于常規(guī)實(shí)驗(yàn)室，也可用在食品檢測(cè)方面。
◆優(yōu)點(diǎn)?特色
● 快速高效提取DNA
● 簡(jiǎn)單操作即可制備PCR的DNA模板
● 成本低
● 用于實(shí)驗(yàn)室和食品檢測(cè)
DNA 提取試劑 CellEase? II 系列產(chǎn)品
◆案例?應(yīng)用
【實(shí)驗(yàn)步驟】
● 實(shí)驗(yàn)前，請(qǐng)先準(zhǔn)備 CellEase? 混合液，按 1：1：3 的比例混合溶液 A、B 和 ddH2O。
● 將樣品切成合適的大小，放入離心管中。
● 向管中加入 CellEase? 混合液。
● 72℃下孵育樣品 6 分鐘。
● 接著在 94℃條件下孵育樣品 3分鐘。
● 即可得到純化 DNA，取適量作為模板，進(jìn)行 PCR實(shí)驗(yàn)。
【實(shí)驗(yàn)案例】
<小鼠尾巴DNA 提取與檢測(cè)>
M ：Marker (100bp ladder)
1?。篊ellEaseII, Extracted solution was undiluted
2?。篊ellEaseII, Extracted solution was ×10 diluted
3?。篊ellEaseII, Extracted solution was ×100 diluted
4 ：Conventional CellEase , Extracted solution was undiluted
5?。篊onventional CellEase , Extracted solution was ×10 diluted
6?。篊onventional CellEase , Extracted solution was ×100 diluted
<全血DNA 提取與檢測(cè)>
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Sample: Whole blood (EDTA) M: Marker (100 bp ladder) 1: CellEase? Blood 2: CellEase? Blood 3: CellEase? Blood 4: Extraction by water |
<金黃色葡萄球菌DNA提取與檢測(cè)>
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M Marker (500 bp ladder) 1: Undiluted Sample Add 5 μl of DNA extract to PCR 2: x 10 dilution 3: x 102 dilution 4: x 103 dilution 5: x 104 dilution |
6: Undiluted Sample Add 6 μl of DNA extract to PCR 7: x 10 dilution 8: x 102 dilution 9: x 103 dilution 10: x 104 dilution |
<番茄葉DNA提取與檢測(cè)>
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M: Marker（500 bp ladder） 1: + CellEase?, Without dilution Add 5 μl to PCR 2: + CellEase?, ×10 dilution Add 5 μl to PCR 3: + CellEase?, × 100 dilution Add 5 μl to PCR 4: – CellEase?, Without dilution Add 5 μl to PCR 5: – CellEase?, ×10 dilution Add 5 μl to PCR 6: – CellEase?, × 100 dilution Add 5 μl to PCR 7: + CellEase?, Without dilution Add 6 μl to PCR 8: + CellEase?, ×10 dilution Add 6 μl to PCR 9: + CellEase?, × 100dilution Add 6 μl to PCR |
10: – CellEase?，Without dilution Add 6 μl to PCR 11: – CellEase?，×10 dilution Add 6 μl to PCR 12: – CellEase?，×100 dilution Add 6 μl to PCR 13: + CellEase?，Without dilution Add 7 μl to PCR 14: + CellEase?，×10 dilution Add 7 μl to PCR 15: + CellEase?，×100 dilution Add 7 μl to PCR 16: – CellEase?，Without dilution Add 7 μl to PCR 17: – CellEase?，×10 dilution Add 7 μl to PCR 18: – CellEase?，×100 dilution Add 7 μl to PCR |
<金槍魚DNA提取與檢測(cè)>
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M: Marker（100 bp ladder） 1: Sample 6 mg, Add 5 μl of DNA extract to PCR 2: Sample 6 mg, Add 6 μl of DNA extract to PCR 3: Sample 6 mg, Add 7 μl of DNA extract to PCR 4: Sample 9 mg, Add 5 μl of DNA extract to PCR 5: Sample 9 mg, Add 6 μl of DNA extract to PCR 6: Sample 9 mg, Add 7 μl of DNA extract to PCR 7: Sample 18 mg, Add 5 μl of DNA extract to PCR 8: Sample 18 mg Add 6 μl of DNA extract to PCR 9: Sample 18 mg Add 7 μl of DNA extract to PCR |
