Beta葡聚糖[混聯(lián)]檢測試劑盒

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β-葡聚糖[混聯(lián)]檢測試劑盒

產(chǎn)品貨號：K-BGLU

產(chǎn)品品名：β-葡聚糖[混聯(lián)]檢測試劑盒

英文品名：β-Glucan (Mixed Linkage) Assay Kit

規(guī)格型號：100 assays per kit

β-葡聚糖是用獨特的工藝開發(fā)的一種新的產(chǎn)品，其來源于新鮮的食品啤酒酵母。它是一種多糖，主要化學(xué)結(jié)構(gòu)β-1，3 葡聚糖和β-1，6葡聚糖，其中前者具有抗腫瘤性質(zhì)，而且能夠極大地提高人體自然免疫力。

上海金畔生物科技有限公司提供β-葡聚糖[混聯(lián)]檢測試劑盒，比色法（510nm）測定谷物、飼料、食品、飲料和其他原料中的B-葡聚糖。

原理：

總檢測時間：100min

檢測限：樣品重量的0.5-100%

適用樣品：燕麥、大麥、麥芽、麥芽汁、啤酒、食品以及其他原料

優(yōu)點：

成本低

提供的所有試劑穩(wěn)定性>2年

只有酶試劑盒可用

特異性高

方法簡單

包含標準品

方法認證：AOAC (Method 995. 16)、AACC (Method 32-23),EBC (Methods 3.11.1,8.16.l 和 8.11. 1)? ICC(標準號l66)，and RACI（標準方法）

分析物意義： 大麥和燕麥的主要細胞壁多糖?

Megazyme檢測試劑盒優(yōu)點：反應(yīng)迅速、試劑穩(wěn)定， 只有酶檢測試劑盒可用。 AOAC方法 995.16; AACC 方法 32-23

AACC Method 32-23.01, AOAC Method 995.16, EBC Methods 3.11.1, 4.16.1 and 8.11.1, ICC Standard No. 166 and RACI standard method for the measurement of 1,3:1,4-?-D-glucan in cereal grains, milling fractions, wort and beer. Recommended/Standard procedure of the Association of Official Analytical Chemists (AOAC), American Association of Cereal Chemists (AACC), Royal Australian Chemical Institute (RACI), European Brewing Convention (EBC), and International Association for Cereal Science and Technology (ICC). Content:100 assays per kit

Colourimetric method for the determination of β-Glucan in
cereal grains, feed, foodstuffs, beverages and other materials

Principle:
(lichenase)
(1) β-Glucan + H2O → β-gluco-oligosaccharides

(β-glucosidase)
(2) β-Gluco-oligosaccharides + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 100 min
Detection limit: 0.5-100% of sample weight
Application examples:
Oats, barley, malt, wort, beer, food and other materials
Method recognition:
AOAC (Method 995.16), AACC (Method 32-23.01), EBC (Methods
3.10.1, 4.16.1 and 8.13.1), ICC (Standard No. 166), RACI (Standard
Method) and CODEX (Type II Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years as supplied
  • Only enzymatic kit available
  • Very specific
  • Simple format
  • Mega-Calc? software tool is available from our website for hassle-free raw data processing
  • Standard included

REFERENCES:

  1. McCleary, B. V. and Glennie-Holmes, M. (1985). Enzymic quantification of (1-3)(1-4)-β-D-glucan in barley and malt.?J. Inst. Brew.?91, 285-295.
  2. McCleary, B. V. and Nurthen, E. J. (1986). Measurement of (1-3)(1-4)-β-D-glucan in malt, wort and beer.?J. Inst. Brew.92, 168-173.
  3. McCleary, B. V. and Codd, R. (1991). Measurement of (1-3) (1-4)-β-D-glucan in barley and oats: a streamlined enzymic procedure.?J. Sci. Fd. Agric.?55, 303-312.
  4. McCleary, B. V. and Mugford, D. C. (1992). Interlaboratory evaluation of β-glucan analysis methods. In “The changing role of oats in human and animal nutrition”. Proceedings of the Fourth International Oat Conference, Adelaide, Australia. Oct 19-23.