乳果糖快速檢測(cè)方法—Megazyme乳果糖快速檢測(cè)試劑盒

Megazyme乳果糖檢測(cè)試劑盒采用酶法分析原理，可定量檢測(cè)鮮奶、UHT奶、濃縮乳和奶粉等各種乳品中的乳果糖。

與農(nóng)業(yè)部新標(biāo)準(zhǔn)采用相同的檢測(cè)原理和方法

該試劑盒采用ISO方法11285：2004，經(jīng)過改進(jìn)后，更加快速和靈敏

靈敏度是傳統(tǒng)己糖激酶法的兩倍

成本低

試劑配制后可穩(wěn)定保存2年

采用試劑盒形式，包含檢測(cè)必需的所有酶

提供計(jì)算軟件，使數(shù)據(jù)處理更方便

包含標(biāo)準(zhǔn)品

The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.

UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products

Principle:
(β-galactosidase)
(1) Lactulose + H₂O → D-galactose + D-fructose

(glucose oxidase + catalase + H₂O₂)
(2) D-Glucose + H₂O + O₂ → D-gluconic acid + H₂O₂

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(4) F-6-P → G-6-P

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP⁺ → ribulose-5-phosphate + NADPH
+ CO₂ + H⁺

Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 120 min
Detection limit: 4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition: Novel method

Advantages

• Twice the sensitivity of traditional hexokinase based lactulose methods
• Very cost effective
• All reagents stable for > 2 years after preparation
• Mega-Calc? software tool is available from our website for hassle-free raw data processing
• Standard included

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