harlan teklad官網(wǎng)動(dòng)物飼料代理商 他莫昔芬飲食 Tamoxifen diets TD.130855

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harlan teklad官網(wǎng)動(dòng)物飼料代理商

他莫昔芬飲食?harlan teklad官網(wǎng)動(dòng)物飼料代理商 他莫昔芬飲食 Tamoxifen diets TD.130855

他莫昔芬激活Creer的飲食

有幾篇文獻(xiàn)引用了含有他莫昔芬的飲食對(duì)CRE模型在雌激素受體突變的控制下的應(yīng)用。恩維戈特克樂(lè)飲食使各種含他莫昔芬的飲食定制，以滿足您的研究需要。我們還制作了各種各樣的含多西環(huán)素飲食對(duì)于被監(jiān)管的系統(tǒng)。如果你有任何問(wèn)題，請(qǐng)咨詢營(yíng)養(yǎng)師。

枸櫞酸他莫昔芬和他莫昔芬

他莫昔芬作為他莫昔芬-蔗糖混合物添加到飲食中.鼓勵(lì)用戶使用庫(kù)存供應(yīng)。然而，泰克樂(lè)將繼續(xù)與客戶準(zhǔn)備的混合。如果您選擇此選項(xiàng)，請(qǐng)咨詢營(yíng)養(yǎng)師，以獲得有關(guān)配制和發(fā)送混合物的更多信息。

鼓勵(lì)用戶從以下庫(kù)存選項(xiàng)中進(jìn)行選擇，以便在幾天內(nèi)隨時(shí)可供裝運(yùn)。

特克樂(lè)提供USP級(jí)三苯氧胺和枸櫞酸他莫昔芬的飲食。
他莫昔芬
每公斤飼料毫克		 日他莫昔芬
每公斤體重毫克1		 飲食編碼
(特克勒德供應(yīng)他莫昔芬)
? ? 天然色 紅色
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400檸檬酸鹽2 40 TD.130859 TD.1308603

1假設(shè)體重20-25克，攝入量3-4克。
2枸櫞酸他莫昔芬為66%他莫昔芬
3在歐洲稱為TAM 400/Creer(TD.55125)

請(qǐng)為輻照飲食增加5-10天的準(zhǔn)備時(shí)間；庫(kù)存和定制產(chǎn)品至少需要3公斤訂單。

這些飼料都有他莫昔芬與~5%蔗糖預(yù)混合，作為一種適口性增強(qiáng)劑；然而，飼料厭惡仍有可能發(fā)生。如果攝入是一個(gè)問(wèn)題，請(qǐng)參閱下面的建議。

TeculadGlobal2016-這些例子中的基礎(chǔ)飲食-僅僅是少數(shù)幾種植物雌激素飲食中的一種。咨詢營(yíng)養(yǎng)學(xué)家關(guān)于其他基礎(chǔ)飲食的使用。

折疊所有標(biāo)簽

  • 最低訂貨量為3公斤，足以喂養(yǎng)20只小鼠一個(gè)月。
  • 儲(chǔ)存冷凍飲食，并計(jì)劃在六個(gè)月內(nèi)使用。
  • 典型的準(zhǔn)備時(shí)間為兩周(如果經(jīng)過(guò)輻照，則為四周)
  • 輻照(可選)20 kGy的最低劑量(必須按順序要求)
  • 訂購(gòu)：請(qǐng)致電800.483.5523或在線啟動(dòng)訂單

計(jì)劃：

  • 最初減少食物攝入量和減肥
  • 日常觀察動(dòng)物的耐受性-咨詢您的IACUC監(jiān)測(cè)指標(biāo)。
  • 必要時(shí)進(jìn)行干預(yù)

如果攝入似乎是一個(gè)問(wèn)題：

  • 通過(guò)進(jìn)行他莫昔芬給藥試驗(yàn)，確定低劑量對(duì)你的模型是否有效。
  • 用含他莫昔芬顆粒與常規(guī)飼料顆?；旌现饾u馴服小鼠
  • 把食物弄濕，然后放在籠子里的盤子里(需要每天更換)。
  • 平日喂飼他莫昔芬飲食，周末有規(guī)律飲食。
  • 每隔一周(三苯氧胺飲食兩周，休息一周)進(jìn)行更長(zhǎng)時(shí)間的治療
  • 他莫昔芬是一種選擇性雌激素受體調(diào)節(jié)劑(SERM)，這意味著它可以抑制雌激素的作用或具有促進(jìn)雌激素的作用。
  • 人體治療效果的一般劑量為20毫克/天。用于嚙齒動(dòng)物的他莫昔芬飲食通常含有~1至2毫克/粒。
  • 當(dāng)處理飲食時(shí)，可以使用典型的實(shí)驗(yàn)室預(yù)防措施，如實(shí)驗(yàn)室外套、手套和面罩，盡量減少接觸他莫昔芬的事故。
  • 您的化學(xué)品安全部門應(yīng)聯(lián)系，以獲得更多的機(jī)構(gòu)特定的指導(dǎo)方針，以處理和處置含有他莫昔芬的飲食。
  • 治療時(shí)間從一周到兩周不等。1,2,5,6,7,8一至兩個(gè)月3,4,8
  • 純他莫昔芬1,5,8和枸櫞酸他莫昔芬1,2,3,4,6,7都是有效的
  • 通常他莫昔芬劑量為每天40-80毫克/公斤體重。
  • 純他莫昔芬的典型包合物為250 mg。8或500毫克1,5枸櫞酸他莫昔芬為每公斤400毫克。1,2,3,4,6,7
  • 報(bào)告的初始重量損失為10%。1,2,4,7,8，與食物攝入量減少有關(guān)2
  • 恢復(fù)正常飲食后體重的恢復(fù)可能會(huì)因基因失活而受損。2,7
  1. 安德森·KB，Winer LH，Mork HK，Molkentin JD，Jaisser F.2010。他莫昔芬誘導(dǎo)CRE介導(dǎo)的小鼠心臟基因斷裂的給藥途徑和劑量。?轉(zhuǎn)基因RES 19：715-725。
  2. 蔣介明，凌杰，鄭永赫，價(jià)格DL，Aja SM，Wong PC.2010年。TDP-43的缺失下調(diào)了與肥胖相關(guān)的基因Tbc1d1，并改變了身體的脂肪代謝。?美國(guó)Acad Sci美國(guó)：16320-16324。
  3. Kardakaris R.，Gareus R.，Xandomlea S，Pasparakis M.2011。內(nèi)皮和巨噬細(xì)胞特異性P38αMAPK缺乏不影響ApoE-/-小鼠動(dòng)脈粥樣硬化的發(fā)病機(jī)制。?PLOSOne 6：e21055。
  4. [2]Kiermayer C，Conrad M，Schneider M，Schmidt J，Brielmeier M.枸櫞酸他莫昔芬口服對(duì)小鼠心臟時(shí)空基因滅活的優(yōu)化。?創(chuàng)世紀(jì)45：11-16。
  5. Koitabashi N，Bedja D，Zaiman AL，Pinto Ym，Zhang M，Gabrielson KL，Takimoto E，Kass DA。2009年。心肌細(xì)胞靶向他莫昔芬誘導(dǎo)MerCreMer基因缺失模型中短暫性心肌病的避免。?中國(guó)保監(jiān)會(huì)第105：12-15號(hào)決議
  6. Kratsios P，Catela C，Salimova E，Huth M，Berno V，Rosenthal N，Morkioti F.2009。細(xì)胞自主Notch信號(hào)在胚胎和成年心臟心肌細(xì)胞中的獨(dú)特作用。?PCirc第106：559-572號(hào)決議。
  7. Miro-Murillo M，Elorza A，Soro-ArNaiz I，Albacete-Albacete L，Ordonz A，Balsa E，Vara-Vega A，Vazquez S，F(xiàn)uertes E，F(xiàn)ernandez-Criado C，Landazuri MO，Aragones J.2011。急性VHL基因失活可誘導(dǎo)心臟HIF依賴的促紅細(xì)胞生成素基因表達(dá).?PLOS One 6：E 22589。
  8. Welle S，Burgess K，Thornton CA，Tawil R.2009。成熟小鼠肌生長(zhǎng)抑素耗竭程度與肌肉生長(zhǎng)的關(guān)系。?李玉玲.醫(yī)學(xué)雜志.1997(4)：E 935-40.

Tamoxifen diets

Diets with tamoxifen activate CreER

Several references cite use of tamoxifen-containing diets for models with Cre under the control of a mutated estrogen receptor.?Envigo Teklad diets makes a variety of tamoxifen-containing diets customized to meet your research needs. We also make a variety of?doxycycline-containing diets?for tet regulated systems. If you have any questions, don’t hesitate to consult a nutritionist.

Teklad stocks tamoxifen and tamoxifen citrate

Tamoxifen is added to the diet as a tamoxifen-sucrose mixture. Users are encouraged to use the stocked supply. However, Teklad will continue to work with customer prepared mixes. If you choose this option, please consult a nutritionist for additional information about preparing and sending the mixture.

Users are encouraged to select from the stocked options below supply readily available to ship within days.

Teklad offers diet with USP grade tamoxifen and tamoxifen citrate
Tamoxifen
mg per kg diet		 Daily tamoxifen
mg per kg body weight1		 Diet code
(Teklad supplied tamoxifen)
? ? Natural colored Red
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400 citrate2 40 TD.130859 TD.1308603

1?Assumes 20-25 g body weight and three-four g intake
2?Tamoxifen citrate is 66% tamoxifen
3?Referred to as TAM400/CreER in Europe (TD.55125)

Please add 5-10 day lead time for irradiated diets; minimum 3 kg orders are required for stocked and customized items.

These diets all have tamoxifen premixed with ~five percent sucrose as a palatability enhancer; however, feed aversion may still occur. If intake is a problem, see below for advice.

Teklad Global 2016 — base diet in these examples — is only one of several minimal phytoestrogen diets Teklad produces. Consult a nutritionist about use of other base diets.

COLLAPSE ALL TABS

  • Minimum order quantity is?three kg, sufficient for feeding ~20 mice for?one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is?two weeks (four weeks if irradiated)
  • Irradiation (optional) minimal dose of 20 kGy (Must be requested at time of order)
  • To place an order: contact Teklad Customer Service at 800.483.5523 or?initiate order online

Plan for:

  • Initial reduction in food intake and weight loss
  • Daily observation of animal tolerance — consult your IACUC for monitoring metrics
  • Intervene when necessary

If intake seems to be a problem:

  • Determine if a lower dose is effective for your model by conducting a tamoxifen dose feeding trial
  • Gradually acclimate mice by mixing tamoxifen-containing pellets with regular feed pellets
  • Wet the food, then place in a dish inside the cage (requires daily replacement)
  • Feed tamoxifen diet on weekdays, regular diet on weekends
  • Alternate weeks (two weeks on tamoxifen diet,?one week off) for longer treatments
  • Tamoxifen is a selective estrogen receptor modulator (SERM) meaning it can repress actions of estrogen or have pro-estrogen effects
  • Usual dose for therapeutic effects in humans is 20 mg/day. Tamoxifen diets for rodents typically contain ~one?to?two mg/pellet
  • Accidental tamoxifen exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of tamoxifen containing diets.
  • Length of treatment varies from one to two weeks1,2,5,6,7,8?to one to two months3,4,8
  • Pure tamoxifen1,5,8?and tamoxifen citrate1,2,3,4,6,7?are both effective
  • Usual tamoxifen doses are ~40-80 mg per kg body weight per day
  • Typical inclusion for pure tamoxifen is 250 mg8?or 500 mg1,5?and for tamoxifen citrate is 400 mg per kg diet1,2,3,4,6,7
  • Initial weight loss of ten % is reported1,2,4,7,8, associated with reduced food intake2
  • Subsequent recovery of body weight after returning to regular diet may be compromised by gene inactivation2,7
  1. Andersson KB, Winer LH, Mork HK, Molkentin JD, Jaisser F. 2010.?Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts.?Transgenic Res 19:715-725.
  2. Chiang PM, Ling J, Jeong YH, Price DL, Aja SM, Wong PC. 2010.?Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism.?Proc Natl Acad Sci USA 107:16320-16324.
  3. Kardakaris R, Gareus R, Xanthoulea S, Pasparakis M. 2011.?Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice.?PLoS One 6:e21055.
  4. Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007.?Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate.?Genesis 45:11-16.
  5. Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA. 2009.?Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models.?Circ Res 105:12-15.
  6. Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. 2009.?Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart.?PCirc Res 106:559-572.
  7. Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011.?Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression.?PLoS One 6:e22589.
  8. Welle S, Burgess K, Thornton CA, Tawil R. 2009.?Relation between extent of myostatin depletion and muscle growth in mature mice.?Am J Physiol Endocrinol Metab.Oct;297(4):E935-40.