harlan teklad官網(wǎng) TD.88051和TD.90221 TD.94059 膽固醇和膽酸鹽的混合高脂飲食
貝弗利·派根和他的同事首先通過(guò)喂養(yǎng)一種混合性動(dòng)脈粥樣硬化飲食來(lái)描述C57BL/6小鼠動(dòng)脈粥樣硬化的發(fā)展。這種混合飼料是通過(guò)將一種天然成分的老鼠飼料按3：1的比例與濃縮的純化飼料(含5%膽固醇和2%膽酸鈉；稱為Thoms-Hartroft飲食)混合而成的。產(chǎn)生的混合物在TD.88051/TD.90221(同配方)含有~15.8%脂肪、1.25%膽固醇和0.5%膽酸鈉。這組人后來(lái)將混合動(dòng)脈粥樣硬化飲食方法與更現(xiàn)代的“西方”純化動(dòng)脈粥樣硬化飲食方法進(jìn)行了比較，并添加了膽固醇和膽酸鹽，發(fā)現(xiàn)混合動(dòng)脈粥樣硬化飲食會(huì)導(dǎo)致更多的膽結(jié)石和肝臟損傷?；旌巷嬍澈卸喾N未精制的成分，這些成分可能會(huì)改變血脂代謝和動(dòng)脈粥樣硬化的發(fā)生，并且不允許對(duì)成分和營(yíng)養(yǎng)物進(jìn)行精確控制，以用于慢性病的研究。雖然已經(jīng)開(kāi)發(fā)出了更精細(xì)的飲食，但混合動(dòng)脈粥樣硬化飲食仍然是野生型小鼠和大鼠誘發(fā)輕度動(dòng)脈粥樣硬化和膽結(jié)石的流行食品。聯(lián)系我們獲得更多的信息，修改，或可能的控制飲食。
添加膽固醇和膽酸鹽的混合高脂飲食實(shí)例*：
添加膽固醇的混合高脂飲食的例子(沒(méi)有膽酸來(lái)源)：
研究用途：
主要在野生型小鼠和大鼠誘發(fā)高膽固醇血癥和輕度動(dòng)脈粥樣硬化(泡沫細(xì)胞、脂肪條紋)。
不會(huì)助長(zhǎng)肥胖。
也用于造石(膽石)嚙齒動(dòng)物的研究。
主要飲食特征：
- 75%的嚙齒動(dòng)物飼養(yǎng)員；25%的純化成分
- 高脂肪(體重約15%；脂肪37%千卡)
- 飽和脂肪酸(SFA>總脂肪酸的45%)
- 膽固醇(1.25%)
- 巧克力源(0.5%)*
參考資料：
- Nishina，下午，J.Verstuyft，B.Paigen，合成低脂和高脂肪飲食，用于研究小鼠動(dòng)脈粥樣硬化。j Lipid RES，1990年。31(5)：P。859-69
- Clee，S.M.，等，血漿和血管壁脂蛋白脂肪酶在動(dòng)脈粥樣硬化中有不同的作用。j Lipid RES，2000年。41(4)：P。521-31
- George，J.等人，用熱休克蛋白-65免疫C57BL/6J小鼠，促進(jìn)了脂肪條紋的形成。Arteroscler Thromb VASc Biol，1999年。19(3)：P。505-10
- Miyake，J.H.，等，膽固醇-7-α-羥化酶基因表達(dá)對(duì)C57BL/6J小鼠動(dòng)脈粥樣硬化的預(yù)防作用。Arteroscler Thromb VASc Biol，2002年。22(1)：P。121-6
- Paigen，B，等，小鼠動(dòng)脈粥樣硬化病變的定量評(píng)估。動(dòng)脈粥樣硬化，1987年。68(3)：P。231-40。
- Schreyer，S.A.，D.L.Wilson和R.C.LeBoeuf，C57BL/6小鼠以高脂飲食作為糖尿病加速動(dòng)脈粥樣硬化的模型。動(dòng)脈粥樣硬化，1998年。136(1)：P。17-24。
- Vergnes，L，等，膽固醇和膽酸組成的動(dòng)脈粥樣硬化飲食誘導(dǎo)不同階段的肝臟炎癥基因的表達(dá)。j Biol Chem，2003年。278(44)：P。42774-84。
*膽酸鈉或膽酸有助膽固醇和脂肪的吸收，并可透過(guò)膽汁酸合成減少膽固醇的排出。然而，如果你的研究不想包括膽酸鹽的來(lái)源，沒(méi)有膽酸鹽的飲食是可用的?？匆?jiàn)TD.96121為了純正的飲食TD.94059混合飲食。請(qǐng)與我們聯(lián)系，了解其他選項(xiàng)。
Hybrid high fat diets with added cholesterol and cholate source*
Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet. The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet). The resulting mixture recreated in?TD.88051/TD.90221?(same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage. Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases. Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats. Contact us for more information, modifications, or possible control diets.
Examples of hybrid high-fat diets with added cholesterol and cholate source*:
Example of hybrid high-fat diet with added cholesterol (without cholate source):
Research Use:
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.
Will not promote obesity.
Also used for lithogenic (gallstone) rodent studies.
Key dietary features:
- 75% rodent breeder diet; 25% purified ingredients
- High fat (~15% by weight; 37% kcal from fat)
- Saturated fatty acids (SFA >45% of total fatty acids)
- Cholesterol (1.25%)
- Cholate source (0.5%)*
References:
- Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69.
- Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis. J Lipid Res, 2000. 41(4): p. 521-31.
- George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65. Arterioscler Thromb Vasc Biol, 1999. 19(3): p. 505-10.
- Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice. Arterioscler Thromb Vasc Biol, 2002. 22(1): p. 121-6.
- Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis, 1987. 68(3): p. 231-40.
- Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis. Atherosclerosis, 1998. 136(1): p. 17-24.
- Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression. J Biol Chem, 2003. 278(44): p. 42774-84.
*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available. See?TD.96121?for a purified diet and?TD.94059?for a hybrid diet. Contact us for additional options.