| 產(chǎn)品編號 | 產(chǎn)品名稱 | 產(chǎn)品規(guī)格 | 產(chǎn)品等級 | 產(chǎn)品價格 |
| 604-21469 | Ampdirect?Plus(INT) | 1ml×5（20μl?體系500?次） | － | － |
| 602-21421 | Ampdirect?PlusBIOTAQ?™?HS?DNA?Polymerase | 1ml×5（20ul?體系500?次）?250?units?（5?units/ul） | － | － |
Ampdirect? Gene Amplification
新型PCR 擴增緩沖液
——無需抽提DNA，直接PCR 擴增！
Ampdirect? Gene Amplification 是一款新開發(fā)的PCR 緩沖液，可有效降低PCR 反應體系里的蛋白質、糖類等各種PCR 抑制物對PCR 擴增的影響。應用廣泛，適合對昆蟲、植物、微生物、土壤、血液、石蠟切片等各類樣品直接進行PCR 擴增。
◆優(yōu)勢
●省時省力省錢
無需DNA 抽提，直接進行PCR 反應，節(jié)省時間
●適用于微量樣品
因為不需要DNA 抽提，不會產(chǎn)生樣品損失，故此最適用于微量樣品
●熱啟動酶
廠家自行開發(fā)的高性能聚合酶BIOTAQ ™
●支持后續(xù)實驗操作
擴增產(chǎn)物可用于后續(xù)的測序和RFLP 等片段分析
◆實驗流程
◆原理
◆示例
注：動物樣本，比如血液和黏膜細胞等可以直接加入PCR 反應液，不需消化處理。固體樣本，比如植物和動物組織需要在含有SDS 和蛋白酶K 的消化液中處理后再進行PCR 反應，或者使用FTA 卡收
集植物組織中的DNA，取帶有DNA 的FTA 卡加入PCR 反應液進行PCR 反應。
◆其他應用：
●血清中病毒檢出
●小鼠尾巴裂解液檢測基因分型
●植物、血液/ 濾紙血液、土壤
●昆蟲、石蠟切片等微量樣品
其他應用的詳細內(nèi)容請看相關資料！
相關資料
◆Ampdirect 推薦使用酶與不推薦使用酶
◆其他應用
Ampdiret 相關的FAQ
◆有關樣品以及樣品前處理
1． 采集后長期保存的血液作為模板，能進行PCR 嗎？
實驗證明，采集后凍存了長達五年時間的血或紙血（干燥血）作為模板，依然能進行PCR。必須長期穩(wěn)定保存血液時，可使用Whatman 公司的FTA Card（核酸保存用卡）進行保存。關于血液（全血）對PCR 的抑制作用，直接采集的血液（新鮮血）對PCR 抑制作用更強。
另外，長久以來的經(jīng)驗證明，采用干燥血作為PCR 模板時，對比用一般的實驗紙保存，使用FTACard 保存的血液進行PCR 的結果更穩(wěn)定，因此推薦使用FTA Card 保存的血作為PCR 模板。
2． 從糞便中抽提的DNA 模板，可用PCR 檢測出細菌嗎？
糞便抽提的DNA 對PCR 有抑制作用，因此用稀釋后的DNA 反應。從糞便樣品中簡便進行PCR 的應用例子，在“使用實時PCR 法高靈敏度檢測Vero Toxin”中有介紹。將10%的糞便懸濁液熱處理（95℃，5 分鐘）后，將離心后的上清液作為PCR 模板。實驗證明，使用這個方法，大部分的糞便樣品都能穩(wěn)定進行PCR。
3． Ampdirect 可應用于RT-PCR 嗎？糞便里存在的RNA 病毒也可以檢測出來嗎？
從糞便樣品中簡便檢測RNA 病毒的應用例子，可見“糞便中的RNA 病毒檢測”。將糞便懸濁液離心后的上清液，涂布在Whatman 公司的FTA Card（核酸保存用卡）上，干燥后，在卡上打（φ1.25mm）就可作為模板使用。
Ampdirect Plus 可做為逆轉錄反應的反應液，逆轉錄酶選用的是Invitrogen 公司的M-MLVReverse Transcriptase（Cat No. 28025-013）。
4． 樣品的溶解處理最后步驟要在95℃，進行5 分鐘的熱處理，這個熱處理步驟是必須的嗎？
因為要使溶解液中的Proteinase K 失活，所以必須熱處理。如果不進行熱處理，Proteinase K 仍保持活性的話，在往PCR 反應液里加入溶解液時，Proteinase K 會將PCR 酶（Taq DNA Polymerase）降解掉，從而不能進行穩(wěn)定的PCR。實際上，這是從大部分得不到目的PCR 產(chǎn)物的例子上取得的經(jīng)驗。所以，必須在最后對溶解液進行熱處理（95℃，5 分鐘）。
5． 從植物的葉片抽提的DNA 作為PCR 模板進行反應，但得不到目的PCR 產(chǎn)物。考慮到是植物里所含的糖類抑制了PCR，使用Ampdirect 可以改善這個情況嗎？
至今為止，已有非常多客戶反映，從實驗植物、谷物、蔬菜、果蔬的葉片取樣，用Ampdirect進行PCR，都取得了良好的結果。不同植物對PCR 的抑制作用也不盡相同，可參照“從植物取樣的簡便PCR 實例”。在PCR 前用溶解液進行前處理。如擔心因為PCR 被抑制而得不到目的PCR 產(chǎn)物，用溶解處理液稀釋5~10 倍，會對此情況有很大改善。
6． 小鼠尾部用溶解液溶解了大約1 個小時，還沒完全溶解。可作為PCR 的模板使用嗎？
將小鼠尾巴1~5mm 浸入100μL 溶解液內(nèi)，55℃下溶解約1 小時后，如小鼠尾巴還未完全溶解，對Proteinase K 進行失活處理（95℃，5 分鐘）后，從溶解液中提取出來的DNA 量（拷貝
數(shù)）應該足以作為PCR 模板，大部分情況下進行PCR 沒有問題。
與此對照，如果是將大條的小鼠尾巴（5mm 以上）過夜處理以至完全溶解，溶解液會變成高粘度狀態(tài)。這種情況下，過剩的DNA 反而會抑制PCR 反應，請用蒸餾水或TE 將溶解液稀釋10 倍左右。請注意盡量不要讓小鼠尾巴過度溶解。
另外，溶解處理后的溶解液不需離心，只需取上清液就可作為PCR 模板使用。從以往經(jīng)驗得知，經(jīng)溶解處理過的溶解液在冷藏保存條件下保存2~3 年內(nèi)皆可作為PCR 模板使用。
7． 不對唾液進行前處理就直接作為PCR 模板是不可行的吧？“取樣自口腔粘膜的PCR”這個應用，是對口腔黏膜細胞用溶解液進行溶解處理，如果是用唾液的話，也必須用溶解液進行溶解處理嗎？
目前，也有不少客戶為了檢測唾液中的微生物，不進行前處理，直接將唾液當成PCR 模板使用。當作為模板進入PCR 反應液的唾液中（數(shù)μL）有大量微生物，可獲得良好的PCR 產(chǎn)物。與之相反，若微生物數(shù)量較少，根據(jù)唾液中的蛋白等物質對PCR 抑制的不同程度，有可能得不到穩(wěn)定的PCR 產(chǎn)物。因此，推薦對溶液用溶解液進行溶解處理，再作為PCR 模板。對唾液進行了溶解處理的話，可得到以下兩種結果，①“抽提微生物的DNA”和②“降低唾液對PCR 的抑制作用（分解唾液中的蛋白）”，也可得到穩(wěn)定的PCR 結果。
8． 如何用PCR 檢測血漿、血清樣品中的微生物？
血漿、血清這些全血，對PCR 有較強抑制作用，所以不能將全血直接用作PCR 模板。因此，要用于“檢測血清中的病毒”時，將血漿、血清用溶解液進行溶解處理，分解掉血漿、血清中的蛋白，降低對PCR 的抑制作用。
請注意，本方法適用于檢測DNA 病毒、細菌，不可用于檢測RNA 病毒。檢測RNA 病毒時，在溶解處理步驟，因為血漿、血清中存在RNA 分解酶，從病毒外殼裸露出來的RNA 就會被分解。
9、使用FTA Card 和實驗紙作為模板進行多樣品PCR 時，因為使用打孔器等工具可能會引起污染。有沒有相應的對策呢？
采集血液樣品時，首次打孔取樣后，在下一次取樣前，用打孔器在FTA Card 或實驗紙上沒有血樣的空白處空打3 個孔，這樣可保證打孔器上不會殘留前一樣品的模板（血樣是白血球DNA）。因為從客戶處得知，“每次用打孔器取樣時，用金伯利（Kimwipe）浸漬酒精擦拭打孔器的打孔部分，這個方法反而引起污染”，所以推薦空打孔的方法。但是，檢測拷貝數(shù)多的DNA，如線粒體DNA 和質粒DNA 等時，本方法不能防止污染。
參考文獻：
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? Zoophilic feeding behaviour of phlebotomine sand flies in the endemic areas of cutaneous leishmaniasis of Sindh Province, Pakistan.
Tiwananthagorn S, et al.
樣品：sand fly（沙蠅）、Leishmania（原蟲）
Fungal Diversity (2012) 52: 49-71
? The sequestrate genus Rosbeeva T.Lebel & Orihara gen. nov. (Boletaceae) from Australasia and Japan: new species and new combinations.
Lebel T, et al.
樣品：菌類、FTA card
Food Chemistry Volume 133, Issue 1, 1 July 2012, Pages 29-37
? Tandem repeat inserts in 13S globulin subunits, the major allergenic storage protein of common buckwheat (Fagopyrum esculentum Moench) seeds.
Khan N, et al.
樣品：蕎麥（種子）
Acta Trop. 2012 Feb; 121(2): 93-8
? Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic.
Fujita M, et al.
樣品：sand fly（沙蠅）
Zool J Linn Soc. 2012 Feb; 164(2): 304-27
? Revision of the Euthalia phemius complex (Lepidoptera: Nymphalidae) based on morphology and molecular analyses.
Yago M, et al.
樣品：昆蟲（鱗翅目）
Limnology (3 November 2011), pp. 1-5
? Surveillance of fish species composition using environmental DNA.
Minamoto T, et al.
樣品：Environmental water
Am. J. Pot Res (2011) 88: 500-10
? Characterization of Crossability in the Crosses between Solanum demissum and S. tuberosum, and the F1 and BC1 Progenies.
Sanetomo R, et al.
樣品：土豆（種子）
Systematic Botany (2011) 36(4): 836-44
? A New Allotetraploid Species of Osmunda (Osmundaceae).
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樣品：蕨類植物（根、葉）
Am. J. Pot Res
? Germplasm Release: Saikai 35, a Male and Female Fertile Breeding Line Carrying Solanum Phureja-Derived Cytoplasm and Potato Cyst Nematode Resistance (H1) and Potato Virus Y Resistance (Rychc) Genes.
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樣品：土豆
Neurochem Res. 2011 Nov;36(11):2127-35.
? Ceruloplasmin protects against rotenone-induced oxidative stress and neurotoxicity.
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樣品：小鼠
Bone. 2011 Nov;49(5):1027-36.
? Insertional mutation in the Golgb1 gene is associated with osteochondrodysplasia and systemic edema in the OCD rat.
Katayama K, et al.
樣品：大鼠
Mycoscience
? The genus Ponticulomyces (Physalacriaceae, Agaricales) from Japan.
Ushijima S, et al.
樣品：菌類、FTA card
Trans R Soc Trop Med Hyg. 2011 Oct;105(10):561-7.
? Leishmania species identification using FTA card sampling directly from patients’ cutaneous lesions in the state of Lara, Venezuela.
Kato H, et al.
樣品：原蟲（Leishmania）、FTA card
Anticancer Res. 2011 Oct;31(10):3607-13.
? Identification of The Distinctive Type i/XhoI+ Strain of Epstein-Barr Virus in Gastric Carcinoma in Peru.
Ordonez P, et al.
樣品：EB病毒
分析手法：PCR-RFLP法
Mol Genet Metab. 2011 Sep 10.
? Newborn screening for Pompe disease in Japan.
Oda E, et al.
樣品：人體血液/濾紙血
Blood. 2011 Sep 29.
? Developmental origins and impact of BCR-ABL1 fusion and IKZF1 deletions in monozygotic twins with Ph+ acute lymphoblastic leukaemia.
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樣品：濾紙血
Mol Biol Evol. 2011 Sep 22.
? Entangling Ancient Allotetraploidization in Asian Mitella: An Integrated Approach for Multilocus Combinations.
Okuyama Y, et al.
樣品：植物
Parasitol Res. 2011 Jul 8.
? Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal of amplification (LAMP).
Laohasinnarong D, et al.
樣品：牛血液、原蟲（錐蟲）
J Agric Food Chem. 2011 Jul 13;59(13):6856-63.
? Practicable group testing method to evaluate weight/weight GMO content in maize grains.
Mano J, et al.
樣品：植物（玉米）
J Parasitol. 2011 Jun 14.
? Molecular prevalence of different genotypes of theileria orientalis detected from cattle and water buffaloes in thailand.
Altangerel K, et al.
樣品：牛、水牛血液、原蟲
Euphytica
? Reciprocal differences in DNA sequence and methylation status of the pollen DNA between F1 hybrids of Solanum tuberosum × S. demissum.
Sanetomo R, et al.
樣品：植物花粉（土豆）
J Vet Sci. 2011 Jun;12(2):191-3.
? A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle.
Higuchi H, et al.
樣品：牛乳、細菌
Biol Pharm Bull. 2011;34(5):779-82.
? Identification of dendrobium species used for herbal medicines based on ribosomal DNA internal transcribed spacer sequence.
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樣品：植物
Zootaxa 2905: 33-56 (3 Jun. 2011)
? A survey of morphological variation in adult Meristogenys amoropalamus (Amphibia, Anura, Ranidae), with a description of a new cryptic species.
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樣品：青蛙
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? Phytogeographic aspects of Lysionotus pauciflorus sensu lato (Gesneriaceae) in the China, Japan and Taiwan regions: phylogenetic and morphological relationships and taxonomic consequences.
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樣品：植物
FEMS Microbiol Ecol. 2011 Jan 11.
? Microbial diversity with dominance of 16S rRNA gene sequences with high GC contents at 74℃ and 98℃ subsurface crude oil deposits in Japan.
Yamane K, et al.
樣品：石油中采集的細菌
J Mol Neurosci. 2011 Feb;43(2):217-24.
? Decreased intake of sucrose solutions in orexin knockout mice.
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樣品：小鼠
Glycoconj J. 2010 Dec 21.
? Excretion into feces of asialo GM1 in the murine digestive tract and Lactobacillus johnsonii exhibiting binding ability toward asialo GM1. A possible role of epithelial glycolipids in the discharge of intestinal bacteria.
Iwamori M, et al.
樣品：細菌（Lactobacillus johnsonii、Lactobacillus casei）
Mycologia. 2010 Dec 21.
? Two species of Strobilomyces (Boletaceae, Boletales), S. seminudus and S. hongoi sp. nov. from Japan.
Sato H, et al.
樣品：菌類
Molecular Ecology Resources. 2010 Oct 28.
? A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus.
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樣品：動物（貓鼬）
Entomological Science 2010 13: 303-310
? An alien Sennertia mite (Acari: Chaetodactylidae) associated with an introduced Oriental bamboo-nesting large carpenter bee (Hymenoptera: Apidae: Xylocopa) invading the central Honshu Island, Japan.
Kawazoe K, et al.
樣品：木匠蜂螨
Vector Borne Zoonotic Dis. 2010 Oct 18.
? Natural Infections of Man-Biting Sand Flies by Leishmania and Trypanosoma Species in the Northern Peruvian Andes.
Kato H, et al.
樣品：原蟲（Leishmania and Trypanosoma Species）
BMC Ecol. 2010 Oct 15;10:21.
? Genetic structure of the oak wilt vector beetle Platypus quercivorus: inferences toward the process of damaged area expansion.
Shoda-Kagaya E, et al.
樣品：昆蟲
Mycoscience. 2010 Oct 30.
? Rapid detection for sporeless trait from Pleurotus pulmonarius culture extracts by using real-time PCR.
Okuda Y, et al.
樣品：菌類
分析手法：融解溫度解析
IBC 2010, vol.2, article no.12, pp.1-7
? Genetic Quality Control of the Rat Strains at the National Bio Resource Project – Rat.
Kuramoto T, et al.
樣品：大鼠、FTA card
分析手法：Amp-FTA method
Int J Environ Res Public Health. 2010 Mar;7(3):814-26.
? Molecular epidemiology for vector research on leishmaniasis.
Kato H, et al.
樣品：昆蟲、原蟲
分析手法：PCR-RFLP法
Am J Sports Med. 2010 Aug 19.
? Cartilage Intermediate Layer Protein Gene Is Associated With Lumbar Disc Degeneration in Male, but Not Female, Collegiate Athletes.
Min SK, et al.
樣品：人口腔粘膜細胞
BMC Evol Biol. 2010 Jun 18;10:185.
? Geographic variation in the damselfish-red alga cultivation mutualism in the Indo-West Pacific.
Hata H, et al.
樣品：植物（紅藻）
J Biochem. 2010 Aug 10.
? NF-kappaB regulates the expression of Nucling, a novel apoptosis regulator, with involvement of proteasome and caspase for its degradation.
Tran NH, et al.
樣品：小鼠
Med Mycol. 2010 Jun;48(4):665-8.
? Nourseothricin acetyltransferase: a new dominant selectable marker for the dermatophyte Trichophyton mentagrophytes.
Alshahni MM, et al.
樣品：真菌
J Plant Res. 2010 May 15.
? Molecular database for classifying Shorea species (Dipterocarpaceae) and techniques for checking the legitimacy of timber and wood products.
Tsumura Y, et al.
樣品：植物
Forensic Toxicology 2010 July Volume 28, Number 2, 1-7
? Chemical constituents and DNA sequence analysis of a psychotropic herbal product.
Kikuchi H, et al.
樣品：植物
J Clin Microbiol. 2010 Aug 18.
? Use of FTA Cards for Direct Sampling of Patients' Lesions in the Ecological Study of Cutaneous Leishmaniasis.
Kato H, et al.
樣品：原蟲、FTA card
Vet Parasitol. 2010 Aug 4;171(3-4):207-15.
? Generation of IFN-gamma-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines.
Yamaguchi T, et al.
樣品：牛血液、原蟲
Jpn J Infect Dis. 2010 May;63(3):173-80.
? Development of triplex SYBR green real-time PCR for detecting Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. without extraction of DNA.
Kerdsin A, et al.
樣品： 細菌 (Mycoplasma pneumoniae、Chlamydophila pneumoniae、Legionella spp.）
分析手法： real-time PCR
Am J Physiol Renal Physiol. 2010 Jun;298(6):F1341-50.
? Phosphaturic action of fibroblast growth factor 23 in Npt2 null mice.
Tomoe Y, et al.
樣品：小鼠
Exp Eye Res. 2010 Jul;91(1):26-33.
? A novel middle-wavelength opsin (M-opsin) null-mutation in the retinal cone dysfunction rat.
Xie B, et al.
樣品：小鼠、FTA card
Am. J. Botany 2010 97: 373-387.
? Developmental morphology of seedling and shoot and phylogenetic relationship of Diplobryum koyamae (Podostemaceae).
Koi S, et al.
樣品：植物
Food Control Volume 21, Issue 5, May 2010, Pages 599-605
? Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor.
Ahmed MU, et al.
樣品：食肉
J Virol Methods. 2010 Feb;163(2):282-6.
? Detection of noroviruses in fecal specimens by direct RT-PCR without RNA purification.
Nishimura N, et al.
樣品：RNA病毒
Theor Appl Genet. 2010 Jan;120(2):205-14.
? DNA methylation in diploid inbred lines of potatoes and its possible role in the regulation of heterosis.
Nakamura S, et al.
樣品：植物（potato）
分析手法：RAPD法
Genes Genet Syst. 2009 Oct;84(5):371-8.
? Comparative differentiation in mitochondrial and chloroplast DNA among cultivated potatoes and closely related wild species.
Hosaka K, et al.
樣品：植物（土豆）
分析手法：PCR-RFLP法
J Fish Dis. 2009 Oct;32(10):857-64.
? Distribution of the introduced cyprinid herpesvirus 3 in a wild population of common carp, Cyprinus carpio L.
Uchii K, et al.
樣品：魚類（鯉魚）、DNA病毒
Plant Physiol. 2009 Dec;151(4):2046-57.
? The phytochrome-interacting factor PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis.
Kidokoro S, et al.
樣品：植物（擬南芥）
Divers. Distrib. 15 (6), 917-927 (2009)
? Chloroplast DNA phylogeography of the endangered Japanese red maple (Acer pycnanthum): the spatial configuration of wetlands shapes genetic diversity.
Saeki I, et al.
樣品：植物（楓樹）
Indian Journal of Science and Technology Vol.2 No. 10 (Oct 2009)
? Development of activated sludge adapted to high concentrations of phenol and enhancement of its phenol removal ability by addition of a processed lignite.
Ohtsuki T, et al.
樣品：活性汚泥
分析手法：PCR-DGGE法
Mol Ecol. 2009 Dec;18(23):4904-11.
? Hybridization involving independent gametophytes in the Vandenboschia radicans complex (Hymenophyllaceae): a new perspective on the distribution of fern hybrids.
Ebihara A, et al.
樣品：植物（蕨類）
分析手法：PCR-SSCP法
Appl Physiol Nutr Metab. 2009 Oct;34(5):926-32.
? Is there a gender difference between ACE gene and race distance?
Min SK, et al.
樣品：人 口腔粘膜細胞
Int J Sports Med. 2009 Sep;30(9):691-4.
? The cartilage intermediate layer protein gene is associated with lumbar disc degeneration in collegiate judokas.
Min SK, et al.
樣品：人口腔粘膜細胞
Biosci Biotechnol Biochem. 2009 Nov;73(11):2452-9.
? Genome-wide identification, structure and expression studies, and mutant collection of 22 early nodulin-like protein genes in Arabidopsis.
Mashiguchi K, et al.
樣品：植物（擬南芥）
Plant Biotechnology 26(4), 435-441, 2009
? Modification of the surface carbohydrate composition of tobacco protoplasts transformed with the human UDP-galactose transporter gene hUGT1
Horibe T, et al.
樣品：植物（煙草）
Genes Brain Behav. 2009 Oct;8(7):650-60.
? A spontaneous mutation of the Wwox gene and audiogenic seizures in rats with lethal dwarfism and epilepsy.
Suzuki H, et al.
樣品：大鼠
J Plant Res 2009; 122: 585-95
? Genetic population structure of Osmunda japonica, rheophilous Osmunda lancea and their hybrids
Yatabe Y, et al.
樣品：植物
Am J Physiol Renal Physiol 2009; 297: F671-8
? Npt2a and Npt2c in mice play distinct and synergistic roles in inorganic phosphate metabolism and skeletal development
Segawa H, et al.
樣品：小鼠
J Immunol 2009;183: 3053-63
? Crucial contribution of thymic Sirp alpha+ conventional dendritic cells to central tolerance against blood-borne antigens in a CCR2-dependent manner
Baba T, et al.
樣品：小鼠
J Nat Med 2009; 63: 340-4
? The botanical origin of kratom (Mitragyna speciosa; Rubiaceae) available as abused drugs in the Japanese markets
Maruyama T, et al.
樣品：植物
分析手法：PCR-RFLP法
Emerg Infect Dis 2009;15: 912-5
? Bartonella quintana in body lice and head lice from homeless persons, San Francisco, California, USA
Bonilla DL, et al.
樣品：虱子、細菌
Plant Cell Physiol 2009; 50: 1579-86
? Arabidopsis bile acid:sodium symporter family protein 5 is involved in methionine-derived glucosinolate biosynthesis
Sawada Y, et al.
樣品：植物（擬南芥）
Mol Genet Metab 2009; 97: 190-5
? High frequency of acid alpha-glucosidase pseudodeficiency complicates newborn screening for glycogen storage disease type II in the Japanese population
Kumamoto S, et al.
樣品：濾紙血
分析手法：ARMS-PCR法
Laboratory Animal research 2009; 25: 75-8
? Simple Genotyping Method Using Ampdirect Plus and FTA Technologies: Application to the Identification of Transgenic Animals and Their Routine Genetic Monitoring
Nakanishi S, et al.
樣品：小鼠-大鼠、FTA card
Int. J. Environ. Res. Public Health 2009; 6: 999-1009
? Association between a Polymorphism of Aminolevulinate Dehydrogenase (ALAD) Gene and Blood Lead Levels in Japanese Subjects
Miyaki K, et al.
樣品：人全血
Jpn J Infect Dis 2009; 62: 164-7
? Direct Colony PCR of Several Medically Important Fungi using Ampdirect(R) Plus
Alshahni MM, et al.
樣品：真菌（酵母?霉菌）
Biochem. Eng J 2009; 45: 76-81
? Characterization of bacterial population of raw milk from bovine mastitis by culture-independent
PCR-DGGE method
Kuang Y, et al.
樣品：牛乳、細菌
分析手法：PCR-DGGE法
Lab Chip 2009; 9: 1052-8
? Microfluidic device using chemiluminescence and a DNA-arrayed thin film transistor photosensor for single nucleotide polymorphism genotyping of PCR amplicons from whole blood
Hatakeyama K, et al.
樣品：人全血
分析手法：PCR-RFLP法
Cancer Cell 2009; 15: 195-206
? Cancer metastasis is accelerated through immunosuppression during Snail-induced EMT of cancer cells
Kudo-Saito C, et al.
Vet Microbiol 2009; 135: 261-6
? Detection of cyprinid herpesvirus 3 DNA in river water during and after an outbreak
Minamoto T, et al.
樣品：河川水、DNA病毒
Methods Mol Biol 2009; 538: 7-27
? Backtracking of leukemic clones to birth
Wiemels J, et al.
樣品：新生兒濾紙血
Int J Plant Sci 2009; 170: 237-46
? Phylogenetic relationship and morphology of Dalzellia gracilis (Podostemaceae, subfamily Tristichoideae)
with proposal of a new genus
Koi S, et al.
樣品：植物
Infect Dis Clin Pract 2008; 16: 230-4
? Association of the SLC11A1 Gene Polymorphisms With Susceptibility to Micobacterium Infections in a Japanese Population
Asai S, et al.
J Vet Diagn Invest 2008; 20: 68-71
? Molecular screening of canine GM1 gangliosidosis using blood smear specimens after prolonged storage:
detection of carriers among shiba dogs in northern Japan
Yamato O, et al.
Biosci Biotechnol Biochem 2008; 72: 2831-9
? Diversity and Similarity of Microbial Communities in Petroleum Crude Oils Produced in Asia
Yamane K, et al.
Vector Borne Zoonotic Dis 2008; 8: 565-73
? Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies
Konnai S, et al.
J. Pestic Sci 2008; 33: 122-7
? Environmental distribution and novel high-throughput screening of APEO-degrading bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)
Ichiki Y, et al.
Zootaxa 2008; 1746: 15-38
? Molecular systematics and biogeography of the genus Zizina(Lepidoptera: Lycaenidae)
Yago M, et al.
Extremophiles 2008; 12: 519-27
? Phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off Shimokita Peninsula
Kobayashi T, et al.
Principles and Technical Aspects of PCR Amplification
? pp91-101, Springer Netherlands, 2008
Elizabeth van Pelt-Verkuil, et al.
Zoolog Sci 2008; 25: 838-42
? Phylogenetic Position of the Endemic Large Carpenter Bee of the Ogasawara Islands, Xylocopa ogasawarensis (Matsumura, 1912) (Hymenoptera: Apidae), Inferred from Four Genes
Kawazoe K, et al.
Mol Phylogenet Evol 2008; 49: 503-13
? Redundant species, cryptic host-associated divergence, and secondary shift in Sennertia mites (Acari: Chaetodactylidae) associated with four large carpenter bees (Hymenoptera: Apidae: Xylocopa) in the Japanese island arc
Kawazoe K, et al.
Odonatologica 2008; 37: 131-44
? Population genetic differentiaiton in three sympatric damselfly species in a highly fragmented urban landscape
Sato M, et al.
Blood 2008; 111: 376-8
? NOTCH1 mutation can be an early, prenatal genetic event in T-ALL
Eguchi-Ishimae M, et al.
Res Vet Sci 2007; 82: 54-60
? Nonsense mutation of feline beta-hexosaminidase beta-subunit (HEXB) gene causing Sandhoff disease in a family of Japanese domestic cats
Kanae Y, et al.
Leuk Res 2007; 31: 1633-40
? Regulatory polymorphisms of multidrug resistance 1 (MDR1) gene are associated with the development of childhood acute lymphoblastic leukemia
Hattori H, et al.
Microbiol Immunol 2007; 51: 507-17
? Plasma levels of unactivated thrombin activatable fibrinolysis inhibitor (TAFI) are down-regulated in young
adult women: analysis of a normal Japanese population
Akatsu H, et al.
Am J Botany 2007; 94: 1630-41
? Cryptic species and host specificity in the ectomycorrhizal genus Strobilomyces (Strobilomycetaceae)
Sato H, et al.
Botanical Journal of the Linnean Society 2007; 155, 1-27
? A global molecular phylogeny of the fern genus Trichomanes (Hymenophyllaceae) with special reference to stem anatomy
Ebihara A, et al.
Trop Anim Health Prod 2007; 39: 369-74
? Comparison of polymerase chain reaction methods for the detection of Theileria equi infection using whole blood compared with pre-extracted DNA samples as PCR templates
Alhassan A, et al.
Am J Trop Med Hyg 2007; 77: 324-9
? Establishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods.
Kato H, et al
Metabolism 2006; 55: 751-7
? G-protein beta 3 subunit polymorphism C1429T and low-density lipoprotein receptor-related protein 5 polymorphism A1330V are risk factors for hypercholesterolemia in Japanese males–a prospective study over 5 years
Suwazono Y, et al.
J Vet Med Sci 2006; 68: 27-33.
? Sequence variation of bovine prion protein gene in Japanese cattle (Holstein and Japanese Black)
Nakamitsu S, et al.
Ann Hum Genet 2006; 70: 767-77
? G-protein beta3 subunit variant C825T is a risk factor for hypertension in Japanese females -a prospective cohort study over 5 years
Suwazono Y, et al.
J Biosci Bioeng 2006; 102: 572-4
? High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction.
Suzuki S, et al
Biosci Biotechnol Biochem 2006; 70: 2387-93
? The molecular phylogeny of the genus Rhizopus based on rDNA sequences.
Abe A, et al
Appl Environ Microbiol 2006; 72: 7912-5
? Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae.
Ohkubo S, et al
Tohoku J Exp Med 2006; 209: 149-57
? G-protein beta3 subunit gene variant is unlikely to have a significant influence on serum uric acid level in Japanese workers.
Suwazono Y, et al
Ornithol Sci 2006; 5: 139-143
? Usefulness of avian buccal cells for molecular sexing.
Arima H, et al
Int J Mol Med 2006; 17: 77-82
? The -1438A/G polymorphism in the 5-hydroxytryptamine receptor 2A gene is related to hyperuricemia, increased gamma-glutamyl transpeptidase and decreased high-density lipoprotein cholesterol level in the Japanese population: a prospective cohort study over 5 years.
Suwazono Y, et al
Arch Virol 2005; 150: 1927-31
? Rotavirus antigenemia in children with encephalopathy accompanied by rotavirus gastroenteritis
Nakagomi T, et al.
Thromb Res 2005; 115: 191-7
? Factor XII Shizuoka, a novel mutation (Ala392Thr) identified and characterized in a patient with congenital coagulation factor XII deficiency
Oguchi S, et al.
Hemoglobin 2005; 29: 1-10
? Hb KOCHI [beta141(H19)Leu–>Val (g.1404C–>G); 144-146(HC1-3)Lys-Tyr-His –>0 (g.1413 A–>T)]: a new variant with increased oxygen affinity
Miyazaki A, et al.
J Epidemiol 2005; 15: 203-10
? Increased risk of obesity resulting from the interaction between high energy intake and the Trp64Arg polymorphism of the beta3-adrenergic receptor gene in healthy Japanese men.
Miyaki K, et al
Br J Pharmacol 2005; 145: 818-28
? Inhibition of the antigen-induced activation of rodent mast cells by putative Janus kinase 3 inhibitors WHI-P131 and WHI-P154 in a Janus kinase 3-independent manner.
Watchara Linwong, et al
Blood Coagul Fibrinolysis 2004; 15: 367-73
? Genetic analyses and expression studies identified a novel mutation (W486C) as a molecular basis of congenital coagulation factor XII deficiency
Ishii K, et al.
Genes Chromosomes Cancer 2004; 39: 335-40
? Protracted postnatal natural histories in childhood leukemia
Maia AT, et al.
Obes Res 2004; 12: 4-8
? Lack of association between human G-protein beta3 subunit variant and overweight in Japanese workers.
Suwazono Y, et al
Drug Matab. Pharmacokin. 2004; 19: 303-7
? Genotyping of single nucleotide polymorphism (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip.
Hiratsuka M, et al
J Vet Diagn Invest 2004; 16: 469-72
? Rapid and simple mutation screening of GM1 gangliosidosis in Shiba dogs by direct amplification of deoxyribonucleic acid from various forms of canine whole-blood specimens.
Yamato O, et al
Blood Coagul Fibrinolysis 2003; 14: 663-70
? Association of the -159 C –> T polymorphism in the CD14 promoter with variations in serum lipoproteins in healthy subjects.
Eilertsen KE, et al.
GENES, Chromosomes & Cancer 2003; 37: 36-43
? Prenatal origin of TEL-AMLI-positive acute lymphoblastic leukemia in children born in California.
McHale CM, et al
Arch Virol 2002; 147: 2187-95
? Molecular characterization of serotype G2 and G3 human rotavirus strains that have an apparently identical electropherotype of the short RNA pattern
Nakagomi T, et al.
BLOOD；2002；99：3801-5
? In utero origin of t(8;21) AML1-ETO translocations in childhood acute myeloid leukemia
Wiemels JL, et al
Proc. Natl. Acad. Sci. USA 2002; 99: 15101-6
? Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia.
Wiemels JL, et al
Clin Lab; 2002; 48: 377-84
? Various applications of direct PCR using blood samples
Nishimura N, et al
Proceedings of the Seventh International Colloquium on Paratuberculosis; 2002; 267-9
? Efficiency of polymerase chain reaction using a novel method of DNA preparation and amplification for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples
Kojima K, et al
Arch Virol 2001; 146: 557-70
? Direct evidence for genome segment reassortment between concurrently-circulating human rotavirus strains
Watanabe M, et al.
Transfusion 2000; 40: 1081-7
? Elimination of both cell-free and cell-associated HIV infectivity in plasma by a filtration/methylene blue photoinactivation system
Abe H, et al.
Microbiol Immunol 2000; 44: 957-61
? Apparent re-emergence of serotype G9 in 1995 among rotaviruses recovered from Japanese children hospitalized with acute gastroenteritis
Oka T, et al
J Clin Microbiol 2000; 38: 2649-54
? Major change in the predominant type of “Norwalk-like viruses” in outbreaks of acute nonbacterial gastroenteritis in Osaka city, Japan, between April 1996 and March 1999
Iritani N, et al
Blood; 2000; 96:264-8
? Detection of clonotypic IGH and TCR rearrangements in the neonatal blood spots of infants and children with B-cell precursor acute lymphoblastic leukemia
Yagi T, et al
Ann Clin Biochem; 2000; 37: 674-80
? Direct PCR from whole blood without DNA isolation
Nishimura N, et al
Stroke 2000; 31: 2661-4
? Polymorphism in the promoter of lipopolysaccharide receptor CD14 and ischemic cerebrovascular disease
Ito D, et al
Stroke 2000; 31: 936-9
? C242T polymorphism of NADPH oxidase p22 PHOX gene and ischemic cerebrovascular disease in the Japanese population
Ito D, et al
Stroke 2000; 31: 493-7
? Association between platelet glycoprotein Iba genotype and ischemic cerebrovascular disease
Sonoda A, et al