L-天門(mén)冬素[天冬酰胺酸]/L-谷氨酸/氨檢測(cè)試劑盒 L-Asparagine/L-Glutamine/ Ammonia 貨號(hào)：K-ASNAM Megazyme中文站

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L-天門(mén)冬素[天冬酰胺酸]/L-谷氨酸/氨檢測(cè)試劑盒

英文名：L-Asparagine/L-Glutamine/ Ammonia

貨號(hào)：K-ASNAM

規(guī)格：100 Assays (50 of each)

分析物意義：  炸、烘、烤土豆或其他食品中的丙烯酰胺的前提

Megazyme檢測(cè)試劑盒優(yōu)點(diǎn)：產(chǎn)品新穎，不到20分鐘可以檢測(cè)3種組分。可用于手工或酶標(biāo)儀自動(dòng)檢測(cè)。

 

The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

UV-method for the determination of L-Asparagine, L-Glutamine 
and Ammonia in potatoes, foodstuffs and cell culture media

Principle:
                               (glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+

                             (microbial glutamate dehydrogenase)
(2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O

                                (asparaginase)
(3) L-Asparagine + H2O → L-aspartate + NH4+

Kit size:                              50 assays of each
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 20 min
Detection limit:                   0.50 mg/L (L-asparagine)
                                           0.54 mg/L (L-glutamine)
                                           0.06 mg/L (ammonia)
Application examples: 
Potatoes, potato products, vegetables, cereals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:            Novel method

Advantages

  • Very rapid reaction due to use of uninhibited glutamate dehydrogenase
     
  • All enzymes supplied as stabilised suspensions
     
  • Only kit available
     
  • Very cost effective
     
  • All reagents stable for > 2 years after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

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